Pritumumab (Biosimilar Reference Antibody) (VIM)
Pritumumab (Biosimilar Reference Antibody) (VIM)
Pritumumab (Biosimilar Reference Antibody) (VIM)-RA0815-Oursmab determined by SDS-PAGE. The purity of the protein is greater than 98%.
ELISA plate pre-coated Antigen protein, can bind Pritumumab (Biosimilar Reference Antibody) (VIM)-RA0815 in a linear range of 31.25–125 ng/mL.
Pritumumab (Biosimilar Reference Antibody) (VIM)

Catalog: 

RA0815

Target

VIM

Isotype

IgG1 - kappa

Applications

ELISA;FC;Functional Assays;IF

Reactivities

Human

Size ($):

100 μg-50;1 mg-210;5 mg-500;10 mg-800;25 mg-1750;50 mg-3000;100 mg-4600

Data Sheet

Material Safety Data Sheet

Certificate of Analysis

Product Description

Pritumumab is a natural, fully human monoclonal antibody (IgG1 kappa) developed as an anti-cancer therapeutic. Its primary mechanism of action is the specific recognition and high-affinity binding to a cell-surface-expressed form of vimentin, particularly the ectodomain variant known as ecto-domain vimentin (EDV). Vimentin, an intermediate filament protein typically located inside cells, can be aberrantly expressed on the plasma membrane of various malignant cells, including those from brain tumors (e.g., glioblastoma), melanoma, and certain carcinomas. By targeting this tumor-associated antigen, pritumumab aims to either directly inhibit tumor cell migration and metastasis, induce antibody-dependent cellular cytotoxicity (ADCC), or potentially deliver a cytotoxic payload if used in a conjugated format.

Synonyms

CLN G11

CAS

499212-74-7

Isotype

IgG1 - kappa

Host

Genetically human

Target

VIM

Reactivity

Human

Expression System

CHO

Applications

ELISA, FC, Functional Assays, IF; Recommended dilution: FC, IF:1:200

Purity

>98% by SDS-PAGE

Concentration

Bottled at the concentration indicated on the vial.

Buffer

0.01M PBS, pH 7.4, contains no stabilizers or preservatives.

Endotoxin

<1.0 EU/mg as determined by LAL method

Conjugation

Non-conjugated

Formulation

Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Storage

store at -20°C or -80°C. Avoid repeated freeze.

Type

Antibody

Purposes

For research use only

References 1

Santosh Kesari, et al. Pritumumab binding to glioma cells induces ADCC and inhibits tumor growth. ASCO Annual Meeting I. 2017.

References 2

Hugwil AV. The meaning of the anti-cancer antibody CLN-IgG (Pritumumab) generated by human × human hybridoma technology against the cyto-skeletal protein, vimentin, in the course of the treatment of malignancy. Med Hypotheses. 2013 Sep;81(3):489-95.

References 3

References 4

References 5

Product Citations 1

Product Citations 2

https://ascopubs.org/doi/abs/10.1200/JCO.2017.35.15_suppl.e14004
https://pubmed.ncbi.nlm.nih.gov/23856243/
https://www.oursmab.com/News/Cellular-cytoskeleton-regulatory-protein-VIM.html

Target Description

Synonyms

vimentin

Biology Area

Cancer Biology;Cell Adhesion/Migration

Function

Vimentin, specifically a conformationally distinct form or epitope exposed on the outer membrane of cancer cells. While vimentin is a canonical intracellular cytoskeletal component involved in maintaining cell shape and motility, its aberrant expression on the cell surface is associated with epithelial-mesenchymal transition (EMT), increased invasiveness, and poor prognosis in many cancers.

Additional Articles 1

Cellular-cytoskeleton-regulatory-protein-VIM

Additional Articles 2

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