Alemtuzumab (Biosimilar Reference Antibody) (CD52)
Alemtuzumab (Biosimilar Reference Antibody) (CD52)
Alemtuzumab (Biosimilar Reference Antibody) (CD52)-RA0124-Oursmab determined by SDS-PAGE. The purity of the protein is greater than 98%.
ELISA plate pre-coated Antigen protein, can bind Alemtuzumab (Biosimilar Reference Antibody) (CD52)-RA0124 in a linear range of 3.91–31.25 ng/mL.
Alemtuzumab (Biosimilar Reference Antibody) (CD52)

Catalog: 

RA0124

Target

CD52

Isotype

IgG1 - kappa

Applications

ELISA;FC;Functional Assays;IF

Reactivities

Human

Size ($):

100 μg-50;1 mg-210;5 mg-500;10 mg-800;25 mg-1750;50 mg-3000;100 mg-4600

Data Sheet

Material Safety Data Sheet

Certificate of Analysis

Product Description

Alemtuzumab is a humanized IgG1 monoclonal antibody targeting the cell surface glycoprotein CD52. It is approved for the treatment of relapsing-remitting multiple sclerosis and B-cell chronic lymphocytic leukemia. Its therapeutic mechanism involves rapid and profound depletion of circulating lymphocytes (T and B cells) and monocytes through antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Following this "immune reset," a distinctive repopulation of the immune system occurs over months to years, which in MS is associated with long-term suppression of disease activity. Treatment requires careful monitoring due to significant risks, including serious infections and secondary autoimmune disorders.

Synonyms

Campath-1H, LDP-03

CAS

216503-57-0

Isotype

IgG1 - kappa

Host

Humanised

Target

CD52

Reactivity

Human

Expression System

CHO

Applications

ELISA, FC, Functional Assays, IF; Recommended dilution: FC, IF:1:200

Purity

>98% by SDS-PAGE

Concentration

Bottled at the concentration indicated on the vial.

Buffer

0.01M PBS, pH 7.4, contains no stabilizers or preservatives.

Endotoxin

<1.0 EU/mg as determined by LAL method

Conjugation

Non-conjugated

Formulation

Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Storage

store at -20°C or -80°C. Avoid repeated freeze.

Type

Antibody

Purposes

For research use only

References 1

Kashani N, et al. Immune Regulatory Cell Bias Following Alemtuzumab Treatment in Relapsing-Remitting Multiple Sclerosis. Front Immunol. 2021;12:706278. Published 2021 Oct 28.

References 2

Yanping Hu, et al. Investigation of the mechanism of action of alemtuzumab in a human CD52 transgenic mouse model. Immunology. 2009 Oct;128(2):260-70.

References 3

References 4

References 5

Product Citations 1

Product Citations 2

https://pubmed.ncbi.nlm.nih.gov/34777337/
https://pubmed.ncbi.nlm.nih.gov/19740383/
https://www.oursmab.com/News/Sialylated-Inhibitory-Receptor-Ligand-CD52.html

Target Description

Synonyms

CD52

Biology Area

CD Markers;Immunology

Function

CD52, a small glycoprotein (12-amino acid peptide) that is highly expressed on the surface of normal and malignant T and B lymphocytes, monocytes, and macrophages, but not on their hematopoietic stem cell precursors. Its physiological function remains incompletely understood. In the context of therapy, the high density of CD52 on target immune cells makes it an ideal antigen for efficient antibody-mediated cell killing. Alemtuzumab's humanized IgG1 isotype optimally engages both complement (CDC) and Fc receptors on effector cells (ADCC), leading to rapid and extensive cytolysis. This results in a prolonged depletion of the adaptive immune cell pool, which, upon reconstitution, is hypothesized to shift towards a more tolerant, less autoreactive state in autoimmune diseases like MS.

Additional Articles 1

Sialylated-Inhibitory-Receptor-Ligand-CD52

Additional Articles 2

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